Hoʻohana ʻo Platelet Dye no Sysmex Hematology Analyzers i ke kemika fluorochrome maʻemaʻe kiʻekiʻe e hāʻawi i ka hoʻopaʻa ʻana i ke kelepona pololei no nā ala ʻike maka. Hoʻopili ka hana i nā membrane cellular i loko o ke keʻena pane, e hoʻopaʻa pono ana i nā waikawa nucleic intracellular ma lalo o ka semiconductor laser excitation. Hoʻemi kēia pane i ka hoʻopilikia ʻana i ka hōʻailona hope me ka mālama ʻana i nā scattergrams optical paʻa ma waena o ka -hoʻokolohua hoʻokolohua kiʻekiʻe.

He aha nā Hematology Stain / Dye Reagents?

ʻO nā mea hoʻoheheʻe hematology automated he fluorochrome solutions i hana ʻia no 5-hapa a me 6-ʻāpana ʻokoʻa anana koko e hana ana me ka semiconductor laser flow cytometry. ʻAʻole like me nā ʻōpala koko microscopic manual, ua hoʻopili kēia mau mea hoʻoheheʻe kemika wai i nā membrane cellular i loko o ke keʻena pane ʻana e hoʻopaʻa pololei me ka intracellular RNA a me DNA. Ma ka hoʻoulu ʻana o ka laser, ʻo ka hopena o ka hoʻokuʻu ʻana o ka kukui fluorescent e ana i ke ʻano cellular, ka nui, a me ka ʻiʻo nucleic acid.

Hana ma ka hoʻokaʻawale ʻana / ʻike

I ka laser flow cytometry, hāʻawi nā mea hoʻoheheʻe fluorescent i ka ʻokoʻa no ka hoʻonā ʻana i nā heluna cell overlapping i nā laʻana koko:
ʻO ka hoʻopaʻa ʻana i ka ʻakika nucleic:Hoʻopili i nā membrane leukocyte a me reticulocyte e hoʻopaʻa i ka RNA intracellular a me nā hale DNA.
ʻO ka ʻokoʻa ʻokoʻa o ka Fluorescence:Hoʻokaʻawale i nā erythrocytes oʻo mai nā reticulocytes a me nā platelets oʻo mai nā hakina platelet i oʻo ma muli o ka nui o ka waikawa nucleic.
Hoʻokaʻawale ʻia nā ʻōpala pūnaewele:Kāohi i ka hoʻopaʻa ʻole-pilikia, pale i nā micro-ʻulaʻula, nā ʻāpana koko ʻulaʻula ʻāpana, a me nā platelets nunui mai ka helu ʻana.
ʻO ka hōʻailona kelepona atypical:Hoʻopuka i nā hōʻailona fluorescence ʻaoʻao ʻokoʻa no ka hoʻokaʻawale ʻana i nā lymphocytes ʻanoʻole, nā pūnaewele pahū, a me nā pūnaʻi koko ʻulaʻula nucleated.

Kaila ʻIke

Pono nā kaha ʻoniʻoni ʻokoʻa i nā mea hoʻoheheʻe fluorochrome kikoʻī e hōʻailona i nā pahuhopu kelepona:
PLT Channel:Hoʻopaʻa i ka RNA intracellular i loko o nā platelets e hoʻokaʻawale iā lākou mai nā ʻōpala erythrocyte a me nā microcytes ma o ka optical fluorescence, e hoʻopau ana i nā hewa helu helu -pili.
Kaila WNR:Hoʻokaʻawale koho i nā keʻena koko ʻulaʻula i ka wā e mālama ana i ka nuclei leukocyte a me nā ʻāpana koko ʻulaʻula nucleated no ka helu like ʻana a me ka helu ʻana.
Kaila WDF:E hoʻopaʻa i ka cytoplasmic a me ka nuclear RNA/DNA o nā neutrophils, lymphocytes, monocytes, a me nā eosinophils e hoʻonā i nā ʻokoʻa leukocyte 5-hapa.
Kaila WPC:Hoʻokaʻawale i nā keʻokeʻo keʻokeʻo maʻamau mai nā cell precursor ʻaʻole e pili ana i ka lipid membrane permeability a me nā ʻano like ʻole o ka nuklea.
Kaila RET:E hōʻailona i ke koena ribosomal RNA i nā erythrocytes oʻo ʻole e helu i nā pākēneka reticulocyte a me nā hakina reticulocyte ʻoi ʻole.

Māhele Analyzer kūpono

XN moʻo	XN-550, XN-1000, XN-2000, XN-3000, XN-9000
XN -L Series	XN-330, XN-350, XN-450, XN-550
XS moʻo	XS-500i, XS-800i, XS-1000i
XT moʻo	XT-1800i, XT-2000i, XT-4000i
XE moʻo	XE-2100, XE-5000

Nā Kūlana Huahana

Mea kiko'ī	ʻĀpana ʻenehana
Code Line Product	STAC -DYE Series
Nā ʻano hoʻohālike	STAC -PAU
Kūlana kino	Haʻina wai maʻemaʻe
Kaila Optical Target	PLT, WNR, WDF, WPC, RET
Ka lōʻihi o ka nalu	633 nm – 635 nm (ʻulaʻula semiconductor laser)
pH hana	6.80 - 7.40 ma 25 degere
Ka helu kānana	0.1 µm kānana wai piha
Hoʻopilikia i ka hōʻailona hope	< 0.1% baseline fluorescence
Palena helu helu	PLT Li'ili'i a i 'ole like me 10 × 10^9/L, WBC Li'ili'i a i 'ole like me 0.10 × 10^9/L, RBC Li'ili'i a i 'ole like me 0.02 × 10^12/L
Ola Paa wehe ʻole ʻia	18 mahina (mālama ʻia ma 2 degere - 35 degere)
Ma ka -Papa Wehe Paʻa	60 lā
Puʻupuʻu-a-Ka ʻokoʻa o ka pūʻulu	CV < 2.0%

Noi & Reagent Hana

Helu PLT
Hoʻopaʻa ka dye Fluorochrome i ka RNA intracellular, hoʻokaʻawale i nā platelets mai nā ʻulaʻula liʻiliʻi a i ʻole nā ʻāpana cell i nā laʻana koko paʻakikī.

5-Mahele Leukocyte Differential (WDF Channel)
Holoi i loko o nā keʻokeʻo keʻokeʻo e hoʻopaʻa i nā ʻakika nucleic, e hana ana i nā ʻano hōʻailona fluorescent e hoʻokaʻawale i nā lymphocytes, monocytes, neutrophils, a me eosinophils.

Hoʻokaʻawale ʻia ke koko ʻulaʻula nucleated (WNR Channel)
Kuhi ʻia nā mea nuklea i loko o ke koko ʻulaʻula nucleated a me nā keʻokeʻo keʻokeʻo i ka manawa hoʻokahi, e pale ana i nā ʻili koko ʻulaʻula nucleated mai ka hoʻonui ʻana i nā helu keʻokeʻo keʻokeʻo.

Kelepona ʻAi ʻole a me ka nānā ʻana i ka pahū (WPC Channel)
Hoʻopili i nā memb lipid o nā pahū ʻai ʻole a me nā cell atypical e hana i nā pūʻulu scattergram ʻokoʻa i ka wā o ka nānā ʻana i ka oncology.

ʻIkepili Reticulocyte (RET Channel)
E hoʻopaʻa i ke koena RNA i loko o nā keʻena koko ʻulaʻula ʻōpio, e hiki ai ke ana i ka hana erythropoietic iwi iwi.

Pūʻolo / Waihona

ʻāpana	Puke a me ka waiho ʻana
Hoʻopiha Volume	12 mL pahu pahu / 42 mL hue
Ipu Kumu	ʻOmole polymer kiʻekiʻe-māmā -palena fluorinated me ke sila akaka
Hoʻopili ʻelua	Kāleka waho ʻōniʻoniʻo me ka hoʻokomo ʻana i ka huʻa haʻalulu
Ka Mahana Waihona	2 degere a 35 degere (Paʻi mai ka lā pololei; mai maloʻo)
Palena Kaʻahele	ʻAʻole pono e ʻoi aku ka ʻike ma luna o 40 degere ma mua o 72 mau hola

Nā mea hoʻopaneʻe hematology pili

ʻO nā mea hoʻoponopono Lyse Automated:Hoʻokumu ʻia nā hopena lysing isotonic no ka hoʻololi wikiwiki ʻana o ka hemoglobin a me ka hoʻoheheʻe erythrocyte.

Nā mea hoʻoheheʻe wai:ʻO nā haʻina electrolyte i hoʻopaʻa ʻia ma ka maʻemaʻe kiʻekiʻe e mālama ana i ka nui o ke kelepona a me ka hoʻokō paʻa.

ʻO ka wai maʻemaʻe:Haʻahaʻa-hopena hoʻoheheʻe laminar no ka hydrodynamically kālele ana nā cell i loko o ka optical flow cell.

Nā mea hoʻomaʻemaʻe Enzymatic:Hana ʻia nā hoʻonā hoʻomaʻemaʻe proteolytic e wehe i nā waihona protein mai nā ala wai.

FAQ

Nīnau: He aha ke ola o ka waihona a me ka paʻa -puʻu hāmama o kēia mau mea hoʻonanea?

A: Mālama ʻia nā ʻōmole i wehe ʻole ʻia he 18-malama ke mālama ʻia ma waena o 2 degere a 35 degere. Ke kau ʻia ma luna o ka mea anaana, hoʻopaʻa ʻia ke kūpaʻa hāmama no 60 mau lā ma lalo o nā kūlana hana maʻamau.

Nīnau: Pehea ka ʻokoʻa ʻana o ka pehu platelet fluorescent optical mai ka helu impedance maʻamau?

A: ʻO ka helu impedance e ana i ka nui o ka cell a hiki ke hoʻopaʻa inoa i nā ʻāpana koko ʻulaʻula microcytic a i ʻole nā palapala cell e like me nā platelets. Hoʻopaʻa ʻia ka waiʻona fluorescent i nā ʻakika nucleic i loko o nā platelets, e hoʻokaʻawale iā lākou ma ka scattergram no ka helu pololei ʻana ma nā laʻana thrombocytopenic koʻikoʻi.

Nīnau: Pono anei nā hoʻololi i ka wā e hoʻokomo ai i kēia mau mea hoʻonanea?

A: Hoʻohālikelike nā ʻano hana kemika me ka osmotic pressure, pH, conductivity, a me ka fluorescence excitation profiles i koi ʻia no nā kahawai optical maʻamau. Hoʻopaʻa ka hoʻomalu maikaʻi maʻamau i ka hana kumu me ka ʻole o ka lako polokalamu a i ʻole ka hoʻoponopono hou ʻana.

Nīnau: Pehea e mālama ʻia ai ka ʻike māmā a me ka wela i ka wā e hele ai?

A: Hoʻoheheʻe ʻia nā mea hoʻonanea ma lalo o ka mālamalama a me nā kūlana maloʻo. Hoʻopili ʻia nā reagents i loko o nā ipu polymer māmā a hoʻouna ʻia i loko o ka ʻeke waho i hoʻopaʻa ʻia e mālama i nā kūlana wela paʻa ma waena o 2 degere a 35 degere.

Nīnau: Pehea e pale ai kēia mau reagents i ka hoʻopili ʻana i ka ʻike o ka hope i loko o nā pūnana kahe?

A: Hoʻohana ʻia nā ʻano hoʻohua i ka -pae 0.1 µm kānana piha a hoʻohana i nā fluorophores ultra-maʻemaʻe. ʻAʻole kēia i ka hoʻohui ʻia ʻana o ka dye a mālama i ka leo fluorescence hope ma lalo o 0.1%.

Nā huahana pale: kala platelet no ka sysmex hematology analyer, Kina platelet dye no sysmex hematology analyers mea hana, mea ho'olako.