ʻO ka WDF Dye no Sysmex 5-Part Hematology Analyzers he mea hoʻomaʻamaʻa fluorescent kūikawā i hoʻokumu ʻia no ka cytometry kahe ʻana o ka optical automated, e komo ana i nā membranes keʻokeʻo keʻokeʻo permeabilized e hoʻopaʻa i nā waikawa nucleic cellular no ka helu ʻokoʻa cell a me ka hoʻokaʻawale scattergram.
He aha nā Hematology Stain / Dye Reagents?
ʻO nā mea hoʻoheheʻe ʻokoʻa hematology dye he mau hoʻonā fluorophore kūikawā i hana ʻia no nā ala ʻike maka i loko o nā - helu helu kelepona. ʻAʻole like me nā ʻili koko lima lima i hoʻohana ʻia i ka microscopy maʻamau, aia kēia mau reagents i nā pūhui fluorescent synthetic i hoʻoheheʻe ʻia i loko o nā mea hoʻoheheʻe kūlohelohe. I ka hoʻomākaukau ʻana o ka cell automated, komo ka reagent i nā cell lysed e hoʻopaʻa i ka RNA intracellular a me DNA, e hana ana i nā hōʻailona fluorescence kikoʻī ma lalo o ka hauʻoli laser.
Hana ma ka hoʻokaʻawale ʻana / ʻike
Hiki i nā mea ho'ohehe'e 'olu'olu ke 'ike i ke 'ano o ke kelepona nui{0}}ma ke ana 'ana i ka ho'opuehu māmā a me ka ikaika o ka fluorescence i loko o ke kele kahe.
ʻO ka hoʻopaʻa ʻana i ka ʻakika nucleic:Hoʻopili ka fluorophore i ka RNA intracellular a me DNA, e hoʻokuʻu ana i ka ikaika māmā e like me ka ʻona nucleic acid a me ka hana metabolic.
Leukocyte ʻokoʻa:Hoʻokaʻawale i nā lymphocytes makua, nā monocytes, nā neutrophils, a me nā eosinophils i mau pūʻulu ʻokoʻa ma nā-manaʻo ʻelua.
ʻO ka hōʻea ʻana o ke kelepona hele ʻole:Hoʻomaopopo i nā lymphocytes atypical a me nā pūnaewele makua ʻole e pili ana i nā hōʻailona fluorescent kiʻekiʻe mai nā kiʻekiʻe RNA a me DNA.
Kaila ʻIke
Hoʻohana ʻia nā ʻano helu helu hematology 5 ʻokoʻa i nā kaila hoʻoheheʻe fluorescent ʻokoʻa no ka hoʻoponopono ʻana i nā heluna pūnaewele kikoʻī:
Kaila WDF:Ke ana i ka helu ʻokoʻa o ke koko keʻokeʻo, nā lymphocytes atypical, a me nā granulocytes makua ʻole me ka hoʻohana ʻana i ka fluorescence ʻaoʻao a me ka hoʻopuehu ʻaoʻao.
Kaila WNR:Nānā i nā helu keʻokeʻo keʻokeʻo a me nā pūnaʻi koko ʻulaʻula nucleated ma o ka hoʻokaʻawale ʻana i nā hōʻailona fluorescence nuklea.
Kaila WPC:Nā pale no nā pūnaewele precursor, nā lymphocytes ʻanoʻole, a me nā heluna cell progenitor.
Kaila RET:Ka helu ʻana i nā reticulocytes a me nā erythrocytes oʻo ma ka huli ʻana i ka RNA ribosomal intracellular.
PLT Channel:Hāʻawi i nā helu platelet optical me ka hoʻohana ʻana i nā māka fluorescent e pale ai i ka hoʻopilikia ʻana mai nā ʻāpana cell a i ʻole microcytic erythrocytes.
Māhele Analyzer kūpono
Mea Analyzers Hematology 5-Part Automated:Nā paepae hoʻokomo kiʻekiʻe -modular, nā ʻāpana kū kaʻawale o ka papahele-, a me nā ʻōnaehana benchtop paʻa.
Nā mea hōʻike huahana kūpono:STAC -DYE-SYSWNR, STAC-DYE-SYSWDF, STAC-DYE-SYSWPC, STAC-DYE-SYSRET, STAC-DYE-SYSPLT
Nā Kūlana Huahana
|
ʻĀpana |
Hōʻike |
|
Huahana Huahana |
STAC-DYE-SYSWDF |
|
Kaila Target |
WDF (Hoʻokaʻawale Koko Keʻokeʻo) |
|
Molekula ʻeleu |
ʻO Polymethine Fluorescent Compound |
|
Ka lōʻihi o ka hawewe |
633–635 nm / 650–670 nm |
|
Ka palena pH |
7.00–7.40 ma 25 degere |
|
pololei (CV) |
WBC Li'ili'i a i 'ole like me 3.0%, NEUT% Li'ili'i a i 'ole like me 5.0%, LYMPH% Li'ili'i a i 'ole like me 5.0% |
|
Kaulike ($R^2$) |
ʻOi aku ma mua a i ʻole like me 0.99 |
|
Ola Paʻa / Paʻa Paʻa Puka Wehe |
18 mau mahina (wehe ʻole ʻia) / 60 mau lā (wehe ʻia) ma 2 degere -35 degere |
Noi & Reagent Hana
ʻO ka ʻokoʻa leukocyte maʻamau 5 ʻāpana:Hoʻopili i nā ʻakika nucleic intracellular e hoʻokaʻawale i nā lymphocytes, monocytes, neutrophils, a me nā eosinophils i loko o nā pūʻulu scattergram ʻokoʻa.
Ka helu ʻana o ka Granulocyte ʻAʻole:Hoʻokumu i nā hōʻailona ʻoi aku ka ikaika o ka fluorescent no nā cell precursor e hiki ai i ka helu ʻakomi me ka nānā ʻole ʻana i ka smear.
ʻIke ʻia ʻo Lymphocyte Atypical:Hoʻomaopopo i nā pae RNA kiʻekiʻe i nā leukocytes reactive e hoʻāla i nā mākaʻikaʻi analytical automated.
Pūʻolo / Waihona
Mahana Waihona:2 degere –35 degere . E kūʻai i loko o nā pahu ʻeleʻele maoli mai ka mālamalama pololei.
Nā kikoʻī o ka pahu:Nā ʻōmole amber polyethylene kiʻekiʻe e hōʻike ana i nā pani pani i hoʻopaʻa ʻia.
Nā Kūlana Kaʻa:Hoʻopaʻa ʻia no ka halihali ukana ambient. E pale i nā kūlana hau ma lalo o 0 degere.
Nā mea hoʻopaneʻe hematology pili
STAC -DYE-SYSWNR:ʻO ka mea hoʻoheheʻe ʻulaʻula no ka helu ʻana o ke koko keʻokeʻo holoʻokoʻa a me ka nānā ʻana o ke koko ʻulaʻula nucleated.
STAC -DYE-SYSWPC:-ʻokoʻa kiʻekiʻe ka mea hoʻoheheʻe fluorescent no ka hoʻopaʻa ʻana i nā leukocyte ma mua.
STAC -DYE-SYSRET:ʻO ka ʻakika nucleic fluorescent no ka helu ʻana i ka hapa hapa reticulocyte.
STAC-DYE-SYSPLT:Optical fluorescent dye reagent no ka hoʻoholo pololei ʻana i ka platelet ma nā laʻana paʻakikī.
FAQ
Nīnau: Pehea e pale ai ka waiʻona WDF i ka hili ʻana o ka hōʻailona ma waena o nā neutrophils a me nā eosinophils ma nā scattergrams?
A: Hoʻohana pū ka hana me nā lyse reagents kūpono e hoʻololi koho i nā membrane cell. Hoʻopili ke kala i loko o ka DNA nuklea a me ka cytoplasmic RNA, e hana ana i ka fluorescence ʻaoʻao ʻokoʻa a me nā ʻaoʻao scatter intensity coordinate no kēlā me kēia heluna cell.
Q: He aha ka lōʻihi o ke kūpaʻa no nā ipu reagent hāmama?
A: E noho paʻa nā pahu hāmama no 60 mau lā ke mālama ʻia ma waena o 2 degere a me 35 degere. Kāohi ka hoʻoheheʻe ʻana i ka hoʻoheheʻe ʻana o ka pūhui ikaika a me ka hoʻoheheʻe ʻana o ka solvent ma lalo o nā kūlana haʻahaʻa haʻahaʻa.
Nīnau: Hiki ke hoʻokomo ʻia kēia mau dye reagents i loko o nā laina wai me ka ʻole o ka hoʻoponopono wai?
A: ʻAe. Mālama ka reagent i ka viscosity fluidic like, nā profile absorption laser, a me nā ʻāpana hana, e ʻae i ka pilina pololei i nā kahawai kahe.
Nīnau: Pehea e nānā ʻia ai ka paʻa fluorescent pūʻulu i ka wā hana?
A: E hōʻoia nā mana chromatography wai i ka maʻemaʻe mole maʻemaʻe no kēlā me kēia pūʻulu hana, a ukali ʻia e ka hoʻāʻo ʻana i ka -kani kahe he nui no ka mālama ʻana i ka neʻe ʻana o ka puʻupuʻu scattergram i loko o ka liʻiliʻi a i ʻole like me 2.0%.
Nā huahana pale: wdf dye for sysmex 5-part hematology analyzers, Kina wdf dye no sysmex 5-part hematology analyrs mea hana, mea hoʻolako.
