I nā makahiki i hala iho nei, me ka hoʻomohala wikiwiki ʻana o ka lāʻau lapaʻau pololei a me ka ʻenehana diagnostic in vitro, ua lilo ka chemiluminescent immunoassay (CLIA) i mea koʻikoʻi i ke kahua o ka maʻi lapaʻau me kona kiʻekiʻe kiʻekiʻe, kiʻekiʻe kikoʻī a me nā pono automation. Ma keʻano he kumu nui o ka pūnaewele CLIA,ʻo ka maikaʻi a me ka hana o ka reagent e pili pono i ka pololei a me ka hilinaʻi o nā hopena ho'āʻo. I ka pane ʻana i kēia koi, makemake nui ka mākeke i nā hopena reagent kūpono a kūpaʻa e hoʻokō i nā pono like ʻole o nā keʻena olakino a me nā laboratories a puni ka honua.
ʻO ka paʻakikī nui o nā mea hoʻoheheʻe immunoassay chemiluminescent pehea e kaulike ai i ka naʻau, kūpaʻa a me ke kumukūʻai. I kēia manawa, ʻo nā ʻenehana reagent koʻikoʻi ka chemiluminescence enzymatic, chemiluminescence pololei a me ka electrochemiluminescence. Ma waena o lākou, hoʻohana nui ʻia ka chemiluminescence enzymatic ma nā mākeke haʻahaʻa -hope a me-hopena ma muli o kāna kumu kūʻai haʻahaʻa a maʻalahi hoʻi; ʻoiai ua lilo ka chemiluminescence pololei a me ka electrochemiluminescence i mea koho nui ma ka mākeke kiʻekiʻe -me ka ʻike kiʻekiʻe a me ka laulā laina ākea. Eia nō naʻe, ʻokoʻa nā reagents o nā ala ʻenehana ʻē aʻe i nā kūlana mālama, ke ola ʻana a me ka hoʻohālikelike ʻana, kahi e kau ai i nā koi kiʻekiʻe ma luna o nā ʻenehana loea o nā mea hoʻolako.
No ka hoʻokō ʻana i kēia mau paʻakikī, ʻo ka ʻoihana alakaʻi -mea hoʻolako hoʻonā reagent ke hoʻoikaika nei i ka hana huahana ma o ka ʻenehana ʻenehana. No ka laʻana, hoʻohana ʻia nā stabilizer kiʻekiʻe a me nā ʻōnaehana buffer e hoʻolōʻihi i ke ola o nā reagents ʻoiai e hōʻoiaʻiʻo ana i ka hana hou a me ka pololei o nā hopena hōʻike. Eia kekahi, ua lilo ka hoʻohālikelike kūpono no nā ʻano like ʻole i ke kī e hōʻoia i ka holo paʻa ʻana o nā reagents ma nā ʻano like ʻole o nā kahua CLIA a hōʻemi i nā deviations ʻike ʻia e nā pilikia pili.
Mai ka hiʻohiʻona o nā ʻano o ka mākeke, ke ulu mau nei ka noi honua no -kiʻekiʻe kiʻekiʻe CLIA reagents, ʻoi aku ka nui o ka nānā ʻana i nā maʻi infectious, ka ʻike ʻana i ka hōʻailona tumo, a me ka maʻi endocrine. I ka manawa like, me ka hoʻolaha ʻana o ka automation a me nā ʻenehana akamai, ke ʻimi nei nā mea hoʻolako reagent i ka hoʻohui hohonu ʻana me nā ʻōnaehana loiloi naʻauao e hoʻomaikaʻi hou aku i ka pono ʻike a me ka hiki ke hoʻokele data.
I ka wā e hiki mai ana, ʻo ka hoʻomohala ʻana i nā reagents immunoassay chemiluminescent e uku nui aku i ka hoʻopilikino ʻana, ka hana kiʻekiʻe a me ka pale ʻana i ke kaiapuni. Ma o ka hoʻomau ʻana i ka ʻenehana a me ka mana koʻikoʻi o ka maikaʻi, e hāʻawi ka ʻoihana i ke kākoʻo hilinaʻi ʻoi aku ka hilinaʻi no ka maʻi lapaʻau honua a kōkua i ka lāʻau lapaʻau pololei e piʻi i kahi kiʻekiʻe.